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Hplc Free Essays

Analysis   and   Separation   of   Organic   Acids   in   White   Wine   Using   High  Performance  Liquid  Chromatography Atis,  Arnelson  Arwin  G. and  Gaitos,  Gerald  M. Institute  of  Chemistry,  University  of  the  Philippines,  Diliman,  Quezon  City   Performed:  December  9,  2011   Submitted:  December  15,  2011 RESULTS  AND  DISCUSSION   solutions   containing   increasing   amounts   of   the   organic   acid. We will write a custom essay sample on Hplc or any similar topic only for you Order Now These   solutions   were   contained   in   flasks   numbered   5,   6,   7,   8,   and   9. (Refer   to   the   Appendix   to   see   components   of   each   flask). Tables   2,   3,   4,   and   5   show  the  peak  areas  and  the  equation  of  the  calibration   curve   obtained   for   each   organic   acid   in   the   chromatograms  obtained  for  flasks  5  to  9. Table  2. Peak  Areas  of  Tartaric  Acid  in  Flasks  5,  6,  7,  8,   and  9   Flask   Retention   Peak  Area   Concentration   time  (min)   (g/L)   5   2. 863   491  364   0. 5   6   2. 905   918  187   1   7   3. 027   5  949  164   5   8   3. 044   8  363  261   8   9   3. 065   9  475  446   10   Calibration  Curve  Equation:  Ã‚   y  =  984  371. 3561x  +  216  064. 755   R2  =  0. 9912      Table  3. Peak  Areas  of  Malic  Acid  in  Flasks  5,  6,  7,  and   8   Flask   Retention   Peak  Area   Concentration   time  (min)   (g/L)   5   3. 02    159742   0. 5   6   3. 760   1  114  051   1   7   3. 756   4  892  727   5   8   3. 795   6  883  424   10   Calibration  Curve  Equation:  Ã‚   y  =  688  260. 8175x  +  475  029. 6892   R2  =  0. 9766      Table   4. Peak   Areas   of   Lactic   Acid   in   Flasks   5,   6,   7,   8,   and  9   Flask   Retention   Peak  Area   Concentration   time  (min)   (g/L)   5   3. 533   575  620   0. 1   6   3. 598   1  248  888   1   7   3. 621   3  762  922   2. 5 The   retention   times   of   different   organic   acids   commonly   found   in   white   wine   were   obtained   using   high   performance   liquid   chromatography. The   peak   areas   of   the   acids   were   then   obtained   through   chromatograms  and  plotted  against  the  concentrations   of   the standard   solutions   of   the   organic   acids   to   construct   a   calibration   curve. Finally,   the   calibration   curves   obtained   were   used   to   determine   the   concentration   of   the   organic   acids   in   samples   of   white   wine. Standard   solutions   of   various   organic   acids   commonly   found   in   white   wine   (tartaric,   malic,   lactic,   citric)   were   assigned   to   be   contained   in   flasks   1,   2,   3,   and   4,   respectively,   and   were   run   through   the   chromatograph. The  mobile  phase  used  for  the  analytes   was   H3PO4   at   pH   3,   to   prevent   dissociation   of   the   organic   acids. The   resulting   chromatograms   of   each   standard   were   then   analyzed   to   obtain   the   retention   times   of   the   organic   acids. Table   1   shows   the   experimental   retention   times   of   the   different   organic   acids. Table   1. Experimental   Retention   Times   of   Organic   Acids   Organic  Acid   Retention  Time  (min)   Tartaric  Ã‚   3. 088   Malic   3. 812   Lactic   3. 620   Citric   3. 68      Since   there   were   no   clear   peaks   from   the   chromatograms   obtained   for   the   tartaric,   malic,   and   lactic   acid   samples,   the   retention   time   at   which   the   peak   height   is   greatest   was   obtained   as   the   experimental  retention  time  of  the  organic  acids. The   experimental   retention   times   were   then   used   to   identify   the   peak   areas   corresponding   to   each   organic  acid  analyzed  in  the  resulting  chromatograms  of 8   3. 597   5  048  614   3   9   3. 619   6  519  896   5   Calibration  Curve  Equation:  Ã‚   y  =  1  300  341. 246x  +  414  396. 3089   R2  =  0. 9815      Table   5. Peak   Areas   of   Citric   Acid   in   Flasks   5,   6,   7,   8,   and  9   Flask   Retention   Peak  Area   Concentration   time  (min)   (g/L)   5   3. 140   361  921   0. 05   6   3. 208   907  232   0. 1   7   3. 306   4  736  427   0. 5   8   3. 325   7  508  481   0. 75   9   3. 347   9  612  454   1   Calibration  Curve  Equation:  Ã‚   y  =  9  836  731. 501x  +  96  328. 12036   R2  =  0. 9994      The   equations   of   the   calibration   curves   were   then   used   to   calculate   the   concentration   of   each   organic   acid   in   the   white   wine   samples. The   peak   area   corresponding   to   each   organic   acid   was   first   obtained,   and   substituted   in   the   calibration   curve   equation. The   concentration  of  each  acid  is  shown  in  Table  6. Table   6. Experimental   Concentration   of   Various Organic  Acids  in  White  Wine  Samples. Organic   Retention   Peak  Area   Concentration   Acid   Time  (min)   (g/L)   Tartaric  Ã‚   2. 966   2  675  651   2. 499   Malic   3. 752   3  576  021   4. 506   Lactic   3. 393   4  709  737   3. 303   Citric   3. 144   794  394   0. 091      The  results  indicate  that  malic  acid  is  the  major   component  of  white  wine. But  in  reality,  this  is  not  the   case. The  major  component  of  white  wine  is  found  to  be   tartaric  acid. The   chromatograms   (See   Appendix)   of   flasks   1   to   9,   and   of   the   sample   are   not   well   resolved. This   discrepancy   may   be   caused   by   several   factors. These   factors   include   poor   solution   preparation,   contamination  of  the  solvent  or  the  sample,  bubbles  in   the   detector,   impurities   in   the   mobile   phase,   bleeding   of  the  column,  inadequate  adjustment  of  equilibrium  in   gradient   operation,   and   carry? over   from   previous   injection. Due  to  these  factors,  it  is  highly  advised  that  the   future   researchers   should   cautiously   execute   each procedure   of   the   experiment   to   eliminate   the   discrepancy   and   accordingly,   they   could   attain   better   results. They   could   also   make   use   of   theoretical   retention  times  of  the  organic  acids  to  determine  each   of   them   and   which   could   further   help   the   future   researchers   to   analyze   the   wine   sample   more   efficiently. REFERENCE:      Meyer,  Veronika  R. Practical  High  Performance  Liquid  Ã‚  Ã‚     Ã‚   Chromatography. 2nd  ed. 1993. England:  John  Ã‚   Wiley  Ã‚  Sons  Ltd. APPENDIX  Ã‚   Data  Sheets  Ã‚      Concentration  of  tartaric  acid  standard:  50. 0  g/L   Concentration  of  malic  acid  standard:  50. 0  g/L   Concentration  of  lactic  acid  standard:  25. 0  g/L   Concentration  of  citric  acid  standard:  5. 0  g/L   Composition  of  Flasks  1? 9   Volume  of  standard  stock  solutions  (in  mL)      Retention  Time  Measurement   Calibrati on  Curve   Flask  #   1   2   3   4   5   6   7   8   Tartaric  Acid   5. 00   0. 00   0. 00   0. 00   0. 25 0. 50 2. 50 4. 00 Malic  Acid   0. 00   7. 50   0. 00   0. 00   0. 10 0. 50 2. 50 5. 00 Lactic  Acid   0. 00   0. 00   5. 00   0. 00   0. 10 1. 00 2. 50 3. 00 Citric  Acid   0. 00   0. 00   0. 00   5. 00   0. 25 0. 50 2. 50 3. 75 9   5. 00 7. 50 5. 00 5. 00 *Chromatograms  of  flasks  1? 9  and  of  the  white  wine  sample  can  be  seen  in  the  remaining  pages  after  this. How to cite Hplc, Papers

John Stuart Mill Essays - Classical Liberalism, Classical Economists

John Stuart Mill After reading 100% of the book, New Ideas From Dead Economists, I chose to write a little summery of John Stuart Mill. I did a little outside research on the subject, because his theories and philosophies were intriguing to me. I was impressed by his change in his views as he entered his mid twenties. John Stuart Mill was born in London on May 20, 1806, and was the oldest son of James Mill. His education, as a boy, was carried out by his father, James Mill. Johns discipline was extremely rigid, as a result, he believed it gave him the intellectual advantage of a quarter century on his contemporaries. Later in life Mill recognized that his fathers extreme system of intellectual discipline gave him little time to develop social and emotional relationships with others. He regretted this aspect of his childhood. Mill was considered a leader in thought at the young age of twenty-one. This is when he encountered a mental crisis. The cause of the crisis, extreme mental and physical strain, gave him as he called it, a dull state of nerves. He realized that the goals in his life, that his father had given him, stolen the feelings out of him. After many months of despair, he found that the emotions within him were not dead. One important factor in this emotional realization was a woman by the name of Mrs. Taylor. She was known to help Mill in authoring works of his, and a good friend. While she was married, Mill held a close relationship with her. After the death of her husband, Mrs. Taylor and John Mill were married in 1851. After this he had great success publishing in multiple literary journals. These articles had ranged from those relating to philosophy and social to political and economic. One of his earliest was with The Westminster Review, but were mostly for The London Review. Through thes e articles, we can trace his gradual development and change in his radical politics. His first real intellectual work appeared in 1843, System of Logic, Ratiocinative and Inductive. This was followed by his, Essays on some Unsettled Questions of Political Economy, in 1844, and, Principles of Political Economy in 1848. In 1859 he wrote On Liberty, and Thoughts on Parliamentary Reform. His Considerations on Representative Government was written in 1860; and in 1863 (after first being published in a magazine) Utilitarianism, one of his most influential writings came to the public. He sat as a member of Parliament from 1865-1868. He advocated three major issues while there; womens suffrage, the interests of the laboring classes and land reform in Ireland. In 1865, came his Examination of Sir William Hamilton's Philosophy. In it, he examines the practical and social aspects of Empiricism and Intuitionism Mill died at Avignon on May 8, 1873. A bronze statue of him stands on the Thames embankment in London, and G.F. Watts copy of his original portrait of Mill hangs in the National Gallery there. He was a man of extreme simplicity as far as his way of life was concerned. His influence upon contemporary English thought cant be overlooked. There has been considerable difference on the longevity of his philosophies Economics Essays

Find a New Career as a Health Care Professional

Find a New Career as a Health Care Professional Sometimes, the desire to be involved in medicine might not strike when you’re young. But then, inspiration hits. Maybe a life-changing event calls you to help others; maybe you realize that after pursuing another career, you have a skill set that lends itself perfectly to the rapidly evolving healthcare field. Even if you amassed a course load (and a degree) that has nothing to do with health care, never fear–it’s never too late for a career change into the growing and thriving medical profession.Your first step? Look for a  postbaccalaureate  program specifically designed to give you the core science classes you missed the first time around that you’ll need for your desired role. (Nurse? Doctor? Veterinarian? Dentist? They’re all up for grabs!)Next, find a mentor. Most programs are designed with career changers in mind and offer a wealth of opportunities to strike out on a path tailor-made for your life. Find a professional you hope to emulate and pick his or her brain. And don’t forget to contact other recent career changers who have been there and can give you advice from the trenches.Finally, pursue this major undertaking with confidence! Whether you’re fresh out of college and want a redo or are decades into your career, many resources are available for you enter the medical field and leave your mark.So you’re thinking of a new career in medicineRead More at USC News

Should Parents Be Held Responsible for the Crimes of Their Children

Should Parents Be Held Responsible for the Crimes of Their Children Who is guilty when teens break the law? Should parents be blamed? Read to know who should be responsible for the crimes of teens. It’s no secret that teens break the law, thinking they’re basically invincible. They engage in underage drinking, vandalism, also possession, and under the influence, of drugs. But then some young people commit much bigger crimes – felonies such as breaking and entering into a house, grand theft auto, even murder, and rape. Children of all ages are capable of breaking the law, but it can be speculated that adolescents tend to break the law more. Unfortunately, teens in American engage in some pretty risky behavior in their middle and high school years. And unfortunately, these crimes have severe consequences that last a lifetime. While these minors are often thought of as children simply making mistakes, they are still rational, mostly reasonable human beings who knowing exactly what they are doing. So, therefore, their crimes should not make their parents responsible. Should Parents Be Held Responsible for the Crimes of Their Children? Could not, for one, be responsible for the crimes of their children, because any person of sound mind is a rational being. They know when they are committing a crime and doing something wrong. It has nothing to do with their parents – unless the parents are condoning, encouraging or taking part in the crime; then, and only then, should parents be held responsible for their children’s crimes. It is rarely the parent’s fault, even if they do teach their children right from wrong. Many people attempt to commit a crime because they think they can get away with it. The same goes for teens committing a crime: They do it, most of the time because they anticipate getting away with it. This is no reflection of one’s upbringing – only the way that person views right from wrong. Even if the children are diagnosed as mentally unsound, insane, crazy, manic-depressive, it’s still not the parent’s fault. SINGLE-PARENT CHILDREN BEHAVIOUR If someone’s child commits a crime and gets caught for it, a parent should not be held responsible for the child because it is the child committing the crime, not the adult. Some may argue how a child’s sense of right and wrong derives from the way their parents raised them – which is true; however, in the case of committing crimes, a child can still steal a pretty piece of jewelry from a friend even if they were taught as a child not to steal. This has nothing to do with the parent, only the child, or teen, committing the crime. If the child commits the crime, they should be at fault and charged appropriately. If one’s child commits the crime, unless the parent directly took part in the illegal activity associated with their child, the parent should without a doubt never be held responsible – not morally, emotionally or legally – for this crime. Parents Are Not Guilty! Unless the parent assisted in helping the child commit the crime –underage drinking drug usage, for example – the adult should never be held responsible for the child’s actions. If a kid gets in trouble, it’s easy to blame the parents, attribute the mishap to how they were raised, the kind of family they were born to. But that is what is wrong with this country: no one takes responsibility for their own actions, especially if they’re minors. It’s a major problem. This way, people become adults never being accountable for anything – they just blame it on someone else. This isn’t the way to go; all it does is condition someone that they are not going to face consequences when the do wrong. Why is that detrimental? Because a person gets a pretty rough reality check as an adult when they are faced with legal charges for committing a crime. At that point, their parents surely aren’t even in the picture. Only the child/teen sho uld get penalized for committing and crime. IS GUN CONTROL AN EFFECTIVE METHOD OF REDUCING CRIME In conclusion, we all make mistakes, and many of us, even on accident, commit crimes (just look at how often people send friends or family members prescription pain-relieve medication. One word: felony). And when people committing crimes are caught, they get charged. The same goes for children, mostly adolescents. They generally know when they are doing something wrong, so they should always be held responsible for their actions – and not their parents. The same, of course, applies to teenagers: when they get caught committing a crime, their parents should never be held responsible unless the parents assisted in the crime. It’s time to hold young people responsible for their actions.

International Trade Policy Case Study Example | Topics and Well Written Essays - 1250 words

International Trade Policy - Case Study Example Development of trade and commerce is essentially linked to the survival, growth, and progress of a nation, particularly by concise means of imports and exports. The policies or regulations that essentially govern and deal with defining the international trade between countries are known as the Foreign Trade Policy or International Trade Policy. These policies include, but are not restricted to, the tax brackets, import duties on various pieces of equipment (goods and services), items for trading, non-allowable items, quotas and tariffs, and so on. Inclusive of this data, there are also the incentives for foreign investment that encourages foreign investors to invest in the country along with incentives for local producers to export, and so on [2]. Thus, the policy defines the terms and regulations for trading i.e. imports and exports. The Asian region has been prospering of late with good speed, and amongst the many nations, Pakistan has been one of the prime examples of prospering nations particularly under the current military regime. The name of this nation has been in the headlines since a while now, thus, it would be very informative to analyze their trade policy structure. Particularly considering the fact that the foreign direct investment has been growing in the region of late, it would be very interesting to see the structure that is enhancing this figure in recent times[3]. Since October 1999 to date, Pakistan has moved towards being a much more stabilized nation than it has ever been since its existence in 1947. Under the military regime of General Pervaiz Musharraf, the nation has moved towards economic stability and growth to the current day, with economic development prospering in particular. This stability in the economic and political environment has led to tremendous growth in the net trade figure, inviting foreign investors and trades to contribute and interact with the local ones and have a good prospectus for the future outlook[5]. This report highlights the trade policy for the year 2006-07 so that judgements can be made on fairgrounds that whether this policy has been a success or otherwise. The following paragraphs highlight the main aims and objectives of the international trade policy relating to the export of goods and services [7, 9].

Why Do We Need Environmental Engineering Topic LOVE CANAL TRAGEDY Case Study

Why Do We Need Environmental Engineering Topic LOVE CANAL TRAGEDY - Case Study Example This led to protests, intervention by the government and multiple lawsuits in pursuit of the compensation for the affected residents. Most of them moved, the infrastructure brought down and the Love Canal crossed for revitalization processes by the Environmental Protection Agency. William T Love is the impresario behind the famous Love Canal as indicated by his last name (Johnson 1). Significantly, the core purpose of the canal was to connect the separations of the Niagara River that had Niagara Falls parting them. Moreover, the canal’s construction would be in a way that residents of the Niagara area would also source electrical power from it; typically, this was a prevalent idea for environmental conservation and community development. Unfortunately, William’s plan faced multiple challenges during the 1892 economic downfall leading to the plan’s ultimate failure; however, this was after he had dug a large percentage of the proposed area. Nonetheless, there was not much that he could do leading to its sale in auction over 25yrs later. Shockingly, the Love Canal served the wrong purposes after its sale. Firstly, the purchasers used it as a chemical disposal site and later on, the United States Army joined the party by using it for warfare experiments that entailed chemical weapons. In the late 1940s, a company (Hooker Corporation) that dealt with chemicals and plastics acquired the Love Canal for the private practices (Phillips 313). Conversely, the company also concealed multiple tons of toxic surpluses in the Love Canal for a period of over 5 years filling it, after which they used land material to cover the chemicals. Niagara City bought the canal to counter its over-population problem from Hooker Corporation that was keen to caveat relative to previous dealings of the land. After purchasing the Love Canal, the city set up infrastructure in form of